Clinical studies & documents

Quantification of Serum Amyloid A in cats

Evaluation of measuring instrument LifeAssays® Vetreader.

Supervisor Dusan Jankovic Leg. Veterinär. Filiz Ibraimi, M.Sc, Chief Operating Officer. Bodil Hernroth, Professor i biomedicinsk laboratorievetenskap, Leg. Biomedicinsk analytiker. Examiner Ann-Sofi Rehnstam-Holm, Professor i Mikrobiologi, Leg. Biomedicinsk analytiker

Abstract Serum Amyloid A (SAA) is an acute-phase protein classified as positive major acute-phase protein (APP),while haptoglobin is classified as positive moderate in cats. Injuries like trauma, infection or inflammation increases the concentration of SAA and haptoglobin in the blood.35 samples from 32 cats was analyzed in this study. Blood from healthy cats and from cats with different disease state were collected. The concentration of SAA (range: 10 –210 mg/L) and haptoglobin (range: 40 –600 mg/dl) were measured in all samples along with the total amount of leukocytes (WBC) and neutrophils (NEU). SAA was measured with LifeAssys ® Feline SAA Test Kit which uses a two-site heterogenous immunoassay. Seven of these samples had a concentration of SAA above the reference (reference: <20 mg/L), while eight had a haptoglobin-concentration above the reference (reference: <250 mg/dl).

Only three samples had simultaneously a concentration of SAA and haptoglobin above reference. Increased quantity of WBC (reference: 2.87 –17.02 *109/L) and NEU (reference: 1.48 –10.29 *109/L) could be seen in conjunction with elevated levels of SAA. A significant positive correlation was observed between SAA and WBC (p-value: <0.001) as well as between SAA and NEU (p-value:<0.001). No significant correlation was obtained between SAA and haptoglobin. Four bloodsamples from a patient with chronic renal failure were collected over eight days. A decrease of SAA-concentration was visibleas a response to treatment. This study shows that Serum Amyloid A (SAA) can be used as an early biomarker for identifying a number of pathological states as well as to monitor how well treatment against an ongoing infection works.

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Acute phase proteins in healthy and sick cats

School of Veterinary Science, University of Queensland, Gatton, QLD 4343, Australia. Research in Veterinary Science (Impact Factor: 1.77). 12/2011; 93(2):649-54. DOI: 10.1016/j.rvsc.2011.11.007 Source: PubMed

ABSTRACT Serum acute phase protein concentrations are used as diagnostic, therapeutic and prognostic markers in human and, less frequently, in animal medicine. The aim of this study was to determine how the health status and signalment of the cat are associated with concentrations of acute phase proteins. Generally, medians of the positive acute phase proteins appeared to be higher in sick cats compared to healthy cats. In multivariable regression models, log-transformed serum amyloid A concentration was higher in older cats, in sick and in female cats, while log-transformed α1-acid glycoprotein and haptoglobin concentrations were higher in older cats and were associated with interactions of health status (sick/healthy) and gender (male/female). The data from healthy cats in this study contribute to the limited knowledge of normal reference ranges for this species. This study highlights the potential of acute phase proteins as diagnostic markers in sick cats, but also emphasises that the signalment of the cat needs to be taken into consideration.

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Evaluation of in vitro diagnostic (point-of-care) system for quantification of the acute phase protein haptoglobin in cats

Jennifer Mattsson, Master's thesis in Biomedical Laboratory Science, Evidensia Animal Hospital, Malmö, Sweden
Spring 2014

This thesis consists of an evaluation of a new in vitro diagnostic point-of-care system for the quantification of the acute phase protein haptoglobin (Feline Haptoglobin, LifeAssays®). Acute phase proteins are biomarkers of systemic inflammation and can thus be used for diagnosis and monitoring of inflammation.1 ...
The results confirm that the method of analysis for Feline Haptoglobin evaluated can be used to identify cats with and without systemic inflammation.

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